Slc5a5^{tm1c(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 71341753 of Chromosome 8 upstream of the critical exons 6 and 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6 and 7 at position 71342751. The critical exons 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. (J:250121)