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Using an sgRNA and CRISPR/Cas9 technology, a FLAG sequence was inserted at the 3' end of the coding sequence immediately upstream of the stop codon. The sgRNA- and Cas9-expressing plasmid and reference plasmid were transfected into EGR-G01 ES cells. The ES cells were then injected into 8-cell ICR embryos. (J:243766)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
