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A loxP site was inserted upstream of exon 1 and an FRT site flanked puromycin resistance gene cassette and a second loxP site into intron 2. The puro cassette was removed through subsequent Flp-mediated recombination. Exons 1 and 2 were deleted through Cre-mediated recombination, creating a null allele. qRT-PCR and immunoblot experiments confirm the lack of expression from this allele. (J:243638)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
