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Two single guide RNA (sgRNA) sequences were designed to delete a portion of exon 4, all of exon 5 and a portion of exon 6 of the gene. Deletion of that region (encoding most of the OTU deubiquitinase domain) creates a premature stop codon in exon 8. No off-target mutations were found (via sequencing the top fifteen off-target candidates for each sgRNA predicted by CRISPR design tool). (J:259730)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
