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A construct comprising the following elements was inserted into the host gene: a loxP site flanked STOP cassette, improved estrogen receptor nuclear translocation domain sequence fused to the cDNA for the fungal I-PpoI homing endonuclease, followed by an IRES and the EGFP fluorescent reporter gene. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette and only after application of tamoxifen will the expressed endonuclease be transported to the nucleus where it targets DNA. (J:249781)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
