A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 1. A second loxP site was inserted into intron 2. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exon 2 floxed. (J:247440)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count