A construct comprising the following elements was inserted into the host locus: CAG promoter, a loxP site, a neomycin resistance gene cassette, a STOP cassette, a loxP site, the mouse gene with a 3' fusion of sequence coding for a hemagglutinin (HA) tag, an FRT site, IRES sequence, the eGFP fluorescent reporter gene, an FRT site, and poly(A) features. The inserted mouse gene and the reporter gene will only be expressed after removal of the neo-STOP cassette through cre-mediated recombination. The reporter gene can be removed through flp-mediated recombination. (J:243119)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Not Specified
Targeted
Insertion
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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