A TRE (Tet response element; 318bp) from pTRE-Tight (Clontech), a GCaMP3 coding sequence (1353bp), a WPRE (609bp), and a SV40 poly A signal (189bp) from pTRE-Tight were inserted into a PCR-generated ROSA26 intron sequences and then subcloned into pKOV-KanF for homologous recombination in E. coli carrying the 200kb BAC clone RP23-401D9. (J:256098)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6N
--
Insertion
--
--
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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