A GFP gene was fused in frame to the C terminus of the gene and a self-excising floxed pgk-neo cre cassette was inserted downstream of the GFP gene. Cre mediate recombination removed the cassette. GFP tagged protein is detected in primary cells by Western blotting and flow cytometry. (J:229465)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count