Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja}

The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), the hVOS1.5 DNA sequence (described below), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. hVOS1.5 is an optimized hybrid voltage sensor consisting of cerulean fluorescent protein (CeFP) with a C-terminal truncated h-ras farnesylation motif that tethers it to the inner face of the plasma membrane. (J:243750)