Kdm5b^{tm1d(EUCOMM)Wtsi}

The L1L2_gt0 cassette was inserted at position 136491938 of Chromosome 1 upstream of the critical exon 6 (Build 37). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 6 at position 136492740. The critical exon 6 is thus flanked by loxP sites. A knock-out allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ/neomycin cassette, followed by cre recombinase to remove the floxed exon 6. (J:223051)