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The full-length mouse cDNA was cloned into the pCDNA3.1(+) vector downstream of the CMV promoter and upstream of the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence. Linearized vector was injected into the pronucleus of fertilized C57BL/6J zygotes. Five transgenic founders (number #14, #50, #55, #63 and #79) were established. The transgene copy number in F1, F2 and F3 generations of founder #50 was determined to be around 17 on average. Western blot analysis showed the expression of the 18 kDa product from the transgene. (J:243722)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
