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Plasmids encoding a signal guide RNA are designed to knock-in a H2B-GFP-pA reporter cassette and a loxP site flanked PGK-NEO cassette into exon 2, the endogenous translational start site of the gene. Cre-mediated recombination removed the floxed neo cassette. Quantitative RT-PCR confirmed that 3' UTR was not amplified although both spliced and unspliced 5'UTR is detected. This is a null allele and a reporter allele. (J:243623)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
