An icre/ERT2 cassette followed by an Frt-flanked neo selection cassette was inserted into downstream of ATG-start codon in frame by homologous recombination in ES cells. Correctly targeted ES cell clones were injected into C57BL/6 blastocyts. Mice were crossed to Flp-expressing mice to delete the neo cassette. (J:201085)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count