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Two sgRNAs (5'-GGTCCGCACCATCGACGGCTCC-3' and 5'-GGAGCTGCGCGAGCGGGTCT-3') were used to target the start of the coding region of exon 1 with CRISPR/Cas9. A number of different deletions were found, with this allele, number 10, representing a 277 bp deletion removing part of the 5' UTR and the start of the coding region (-180 to +97). The absence of transcripts from this allele was confirmed by qPCR and the absence of peptides by Western blot analysis of thymus and brain samples. (J:241384, J:243187)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
