Cfap157^{tm1d(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 32671182 of Chromosome 2 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 32671954. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. RT-PCR, northern blot and immunoprecipitation experiments confirmed the lack of wild-type expression from this allele. qRT-PCR did detect transcripts lacking exon 2 or exon 2 and 3, but no peptides were detected. (J:237484)