Gucy1b1^tm1.1Brou

Exon 5 was replaced with a mutant version carrying an H105F point mutation and a floxed pgk-neo cassette was inserted downstream of exon 5 via homologous recombination. Cre-mediated recombination removed the neo cassette. The replacement of the wild-type histidine with a phenylalanine interferes with haem ligation resulting in loss of NO activation. (J:227198)