A loxP site was inserted in intron 2 and a loxP and FRT flanked neo cassette were inserted in intron 4 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:227203)
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A loxP site was inserted in intron 2 and a loxP and FRT flanked neo cassette were inserted in intron 4 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:227203)