A KOMP (Aclytm184180a(L1L2_Bact_P)) targeting vector containing a FRT-lacZ-loxP-neo-FRT-loxP cassette was introduced to intron 8, and a loxP site was placed in intron 9 via homologous recombination. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 9 floxed. (J:240494)