Hhex^tm2.1Jomb

The starting point was allele Hhextm1Jomb which is constructed as follows. The following elements were inserted into exon 1 after the ATG start codon: the DsRed.T3 (enhanced T3 variant of Red Fluorescent Protein, also called RedStar) gene, an IRES, a blasticidin resistance gene (BSD), a polyA signal and a loxP site flanked hygromycin resistance gene cassette. From this allele the blood enhancer element (Rr137) in intron 1 was deleted. The hygro cassette was removed through subsequent cre-mediated recombination. Fluorescence microscopy and FACS analysis confirms the lack of expression from this allele in the hematopoietic system. (J:241018)