Crkl^{tm1d(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 17286305 of Chromosome 16 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 17287491. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created cre recombinase expression in mice carrying this allele to remove the loxP-flanked critical exon 2. (J:242224)