Igdmr^{em1(Snrpn-EGFP)Jae}

CRISPR-Cas9 methodology is used to introduce a knock-in DNA methylation reporter (Snrpn*/GFP) adjacent to the 3' end of the Dlk1-Dio3 locus on mouse chromosome 12 (the IG-DMR, intergenic differentially methylated region).The Snrpn*/GFP cassette contains a GFP fused to the minimal promoter region of the mouse Snrpn gene; it includes conserved elements and the endogenous imprinted differentially methylated region. Maternal inheritance of the reporter allele is required to express the GFP reporter. (J:239124)