A loxP site is inserted in intron 2 and an FRT-loxP-neomycin-FRT-loxP cassette was introduced to intron 3. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:241281)
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A loxP site is inserted in intron 2 and an FRT-loxP-neomycin-FRT-loxP cassette was introduced to intron 3. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:241281)