A LacZ-neo resistance cassette replaced 246 bp of the gene via homologous recombination deleting exon 3. qPCR analysis using a probe spanning exon 2/3 boundary failed to detect product. (J:199257)
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A LacZ-neo resistance cassette replaced 246 bp of the gene via homologous recombination deleting exon 3. qPCR analysis using a probe spanning exon 2/3 boundary failed to detect product. (J:199257)