The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion starting at position 135566138 and ending at position 135566339 of Chromosome 7 (Genome Build 38). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and an FRT site. (J:174268)