Smc3^{tm1c(EUCOMM)Wtsi}

The L1L2_gt1 cassette was inserted at position 53603082 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 53603827. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:228976)