A neomycin resistance cassette flanked by both FRT and loxP sites was inserted upstream of exon 4 and a third loxP site downstream of exon 5. The neo cassette was excised in the germline by flp recombinase. (J:240861)
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A neomycin resistance cassette flanked by both FRT and loxP sites was inserted upstream of exon 4 and a third loxP site downstream of exon 5. The neo cassette was excised in the germline by flp recombinase. (J:240861)