The transgene construct consisting of truncated CAG promoter and FRT-flanked modified GFP/neo fusion gene followed by CreMer fusion protein and truncated Pgk poly-A was inserted into the intron 3 of Rprd1b gene. Prior to FLP-mediated excision of the FRT-flanked GFP/neo fusion gene, expression of GFP/neo is observed in various tissues. By breeding to Flip recombinase expressing mice, Rprd1b transgenic line was generated by removing GFP/neo fusion gene. (J:240153)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count