Nradd^tm1.2Pabk

LoxP sites were inserted to flank exon 2. A frt-flanked neomycin selection cassette was removed via Flp-mediated recombination, and subsequent Cre-mediated recombination deleted exon 2 to generate the null allele. Western blot analysis confirmed the absence of protein expression in spleen tissue of adult homozygous mutant mice. (J:237623)