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BAC RP11-72J8 was modified to include a 982bp synthetic gene that includes 500 bp of ROSA26 sequence to allow in vivo linearization by the CRISPR/Cas9 complex. Specifically, the gene contains from 5' to 3': human SLC10A1 sequence, an FRT-flanked PGK-Neo cassette, 500 bp of ROSA26 sequence and human SLC10A1 sequence (SLC10A1 sequence totals 397 bp). Sequence analysis indicates that the ROSA26 sequence is 76,428 bp upstream of exon 1 of the SLC10A1 gene. Single guide RNAs are designed to target the ROSA26 locus and the RP11-72J8 BAC. (J:263861)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
