Brca1^{tm1.2(BRCA1*)Tsr}

A targeting vector containing (5' to 3'): human BRCA1 exons 2 to 14 - loxP site - F3 mutant FRT site - Puromycin cassette - F3 mutant FRT site - human BRCA1 exons 15 to 23 - mouse Brca1 3' UTR - HGHpA - loxP site - human mutant BRCA1 exons 15 to 23 (including the 5382insC point mutation in exon 19) - mouse Brca1 3' UTR - HGHpA - FRT - NEO cassette - FRT, was used to replace the coding region in exon 2 and the splice donor-site in intron 2 of the mouse Brca1 gene.The 5382insC point mutation inserts a cytosine at position 5382 resulting in a premature translation initiation codon in exon 23. Transient flp-mediated recombination removed both the puro and neo cassettes. Cre-mediated recombination removes human wild-type BRCA1 exons 15-23 and allows transcription of the 5382insC mutation. (J:240315)