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A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 1. mRNA expression levels were studied through RT-PCR and determined that the neo cassette interfered with the transcription of the allele. Therefore the neo cassette was removed through subsequent flp-mediated recombination. Exon 1 was deleted by subsequent cre-mediated recombination, creating a null allele. RT-PCR confirmed depletion of transcripts from this allele. (J:239934)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
