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Exon 3 was targeted with the CRISPR/Cas9 system. The sgRNA sequence was 5'-CACCTAGCCTCGCACGAActt-3' (recognition sequence in upper case) and the experiment resulted in a 5 bp deletion (CTCGC) in the middle of the recognition sequence. The resulting frameshift introduces a premature stop codon soon after, knocking the gene out. Western blots confirmed the absence of peptides translated from this allele in homozygous mice. (J:239003)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
