An FRT site flanked neomycin resistance gene cassette and loxP sites flanking exon 4 were inserted. The neo cassette was removed through subsequent flp-mediated recombination and exon 4 was deleted through cre-mediated recombination. (J:237765)
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An FRT site flanked neomycin resistance gene cassette and loxP sites flanking exon 4 were inserted. The neo cassette was removed through subsequent flp-mediated recombination and exon 4 was deleted through cre-mediated recombination. (J:237765)