A loxP site was inserted into intron 5; an FRT site flanked neomycin resistance gene cassette and the inverted cDNA for the human gene followed by an inverted splice acceptor site and an inverted loxP site were inserted into intron 6. Cre-mediated recombination inverted the sequence between the loxP sites and removed the selection cassette. RT-PCR confirmed expression of the transgene from this allele. (J:238133)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
Insertion
--
1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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