H2-Ab1^b-tm2Hpl

The endogenous class II MHC locus was replaced by a construct that encodes a mutant class II MHC beta-chain that cannot be ubiquitinated (K225R). A construct was used in which the long arm of homology corresponds to exons 24 of the I-Ab locus, followed by fused exons 5 and 6, in frame to the coding region for EGFP. The K225R mutation in the cytoplasmic tail of class II MHC was introduced into exon 4 of this construct. The construct contained a self-excisable ACN cassette that consists of a neomycin resistance gene, and a gene for Cre recombinase under the control of a sperm-specific promoter flanked by loxP sites. Transcriptional induction of the ACN cassette resulted in the excision of the neomycin resistance gene and Cre recombinase when the gene is transmitted through the male germline. (J:238328)