The targeting vector is designed to insert loxP sites flanking the entire coding region. A FRT-flanked neomycin resistance (neo) cassette was inserted upstream of the 3' loxP site. Cre-mediated recombination removed the coding region. (J:238262)
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The targeting vector is designed to insert loxP sites flanking the entire coding region. A FRT-flanked neomycin resistance (neo) cassette was inserted upstream of the 3' loxP site. Cre-mediated recombination removed the coding region. (J:238262)