A loxP site flanked neomycin resistance gene cassette was inserted into the genome, replacing the target gene, thus creating a null allele. The neo cassette was removed through cre-mediated recombination. (J:237895)
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A loxP site flanked neomycin resistance gene cassette was inserted into the genome, replacing the target gene, thus creating a null allele. The neo cassette was removed through cre-mediated recombination. (J:237895)