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Exons 3 and 4 of the targeted gene were replaced with a construct containing: loxP-FRT-polyA-neomycin (in reverse transcriptional orientation)-FRT-exons 3-9 of Pofut2 mini cDNA-polyA-loxP-Stop-mCherry-polyA. Flp-mediated recombination removed the FRT-flanked neo cassette. mCherry fluorescence is not detectable in this allele. Cre-mediated recombination removed the loxP-flanked exons 3-9. In-situ hybridization and H&E staining indicates that embryos fail to express markers of extra-embryonic ectoderm and mesoderm differentiation. (J:234992)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
