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This transgene contains the tetracycline-responsive P-tight promoter (from the plasmid pTRE-Tight, BD Biosciences Clontech), which comprises a modified Tet-responsive element containing seven direct repeats of a 36-bp sequence including the 19-bp tet operator sequence followed by a minimal cytomegalovirus (CMV) promoter, driving expression of a human tumor necrosis factor alpha cDNA whose 3' untranslated region (3'-UTR) has been replaced by the SV40 polyadenylation signal. This transgenic line was estimated by Southern blot analysis to carry 5 tandem copies of the transgene. No human TNFalpha is detected by enzyme-linked immunosorbent assay (ELISA) in sera of doxycycline-treated or untreated mice bearing this transgene alone. (J:236003)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
