Tg(CMV-Cux1)#Gbvh

This transgene comprises the cytomegalovirus (CMV) promoter, a 6564-base pair mouse Cux1 cDNA containing 27 bp of 5' untranslated region (5'-UTR), the 4544 bp protein-coding sequence and 1993 bp of 3'-UTR, and the SV40 small t intron and polyadenylation signal. CUX1 protein is detected at low levels in nuclear extracts of kidney and spleen of adult wild-type mice and at high levels in kidney, liver, spleen and testis of transgenic mice by immunoblot analysis using a goat polyclonal antibody to a 19-amino acid N-terminal peptide of CUX1, which is not present in the truncated testis-specific endogenous CUX1 protein isoform. Immunohistologic analysis reveals ectopic expression in capillary loop-stage and maturing glomeruli of transgenic embryos and in both glomeruli and tubular cells of adult transgenic mice. (J:76261)