Tg(Kap)L49Msgr

In male mice with this transgene, expression of cDNA encoding the mouse kidney androgen regulated protein (KAP) is driven by the mouse Kap promoter and the distal regulatory sequences of the human angiotensinogen gene (AGT), which together confer androgen responsiveness. The Kap cDNA was cloned into the NotI site of the pKAP2 vector, downstream of Kap nucleotides -1542 through -1 (relative to the transcription initiation site) and followed by the 3' 42 bp of exon 2 (which excludes the translation initiating ATG codon and most of the coding sequence) through exon 5 of human AGT. RT-PCR and western blot analysis of samples from various tissues detects no expression in tissues other than kidney and demonstrates approximately twice the level of both mRNA and protein in kidneys of 6-8 month old male transgenic vs. control mice. Immunohistochemical analysis using an anti-KAP monoclonal antibody showed the protein to be restricted to the proximal tubule cells of the kidney. However, at higher magnification "diffuse, intense staining" was apparent throughout transgenic male mice; control males exhibited less intense general staining. (J:83177, J:95430, J:166496)