A minigene containing the coding sequence of the inserted gene was coupled to genomic sequence containing the last three exons (3' part of exon 9 through entire exon 11) of the host gene. This combined sequence followed by a loxP-flanked neomycin resistance gene cassette was inserted at the ATG translation start site of the host gene. Subsequent cre-mediated recombination removed the neo cassette. Experiments confirm the functional expression of the inserted gene. This allele was generated as an experimental control, the inserted gene being the same as the host gene. (J:234164)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/SvImJ
Targeted
Duplication, Insertion
--
1
8
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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