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A sequence was engineered comprising the tdTomato red fluorescent protein open reading frame followed by the SV40 polyadenylation sequence and an FRT site-flanked neomycin resistance cassette. This was inserted into the BAC RP23-407K8 immediately downstream of the translation-initiating ATG in exon 1 of the mouse glutamic acid decarboxylase 2 gene (Gad2; also called Gad65); the BAC contains all 16 exons, 72 kb of 5'- and 64 kb of 3'-flanking DNA. The neo cassette was removed by flp-mediated recombination. Of 11 founder mouse lines that had integrated the transgene and that transmitted it through the germline, only one exhibited the expected expression pattern upon fluorescence imaging of brain sections. (J:233808)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
