CRISPR/Cas9 technology inserted between the 5' UTR and the exon 1 coding sequence a lacZ reporter gene with a nuclear localization sequence, a polyadenylation sequence and an FRT-flanked neomycin resistance cassette. Clones 4 (13 bp deletion), 6 (6 bp deletion), 11 (162 bp deletion), 12 (26 bp deletion), 14 (no deletion) and 17 (7 bp deletion) were generated. Clones 5 and 11 (represented by #) are positive for lacZ insertion. (J:232970)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x DBA/2)F1 x C57BL/6J
Endonuclease-mediated
Insertion, Intragenic deletion
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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