CRISPR/Cas9 technology inserted between the 5' UTR and the exon 1 coding sequence a lacZ reporter gene with a nuclear localization sequence, a polyadenylation sequence and an FRT-flanked neomycin resistance cassette. Clones 4 (13 bp deletion), 6 (6 bp deletion), 11 (162 bp deletion), 12 (26 bp deletion), 14 (no deletion) and 17 (7 bp deletion) were generated. Clones 5 and 11 (represented by #) are positive for lacZ insertion. (J:232970)
Basic Information
(C57BL/6 x DBA/2)F1 x C57BL/6J
Insertion, Intragenic deletion
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count