A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 3. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. (J:228948)
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A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 3. Flp-mediated recombination removed the selection cassette and left exon 2 floxed. (J:228948)