Tg(CD2-Tcra,-Tcrb)F8Mack

Tcra (Valpha8/Jalpha24/Calpha) and Tcrb (Vbeta13/Dbeta2/Jbeta2/Cbeta2) cDNAs were amplified from Sur20-28 tetramer binding CD8+ splenocytes and cloned into human CD2 minigene-based vectors. Sequence was introduced between (on the 5' end) the promoter, ATG-free first exon and first intron of the human CD2 gene and, on the 3' end ~500 bp of the 3' untranslated and flanking DNA of human CD2, including two polyadenylation signals and the 3' locus control region. The two vectors were coinjected and three founders (E8, F8, L8) with a high frequency of Sur20-28 tetramer-binding CD8+ T cells were selected. The F8 transgene integrated on mouse Chromosome 11. Mice express a TCR with specificity for murine Birc5 (survivin) peptide (20-28) presented by H2. (J:222268)