Tg(tetO/Prnp-GLRA1*,-EYFP)9542Jjan

The transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). Lines 9531 and 9542 displayed the strongest signal. Line 9531 is recommended by the donating investigator for most experimental purposes as it displays less tTA-independent expression in the cerebellum than line 9542. (J:232624)