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The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT-flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-frt-Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. Flp-mediated recombination removed FRT-flanked STOP cassette. (J:235493)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
