The targeting vector is designed to insert a single loxP site upstream of exon 1, and a loxP-flanked and FRT-flanked neomycin resistance (neo) cassette downstream of exon 1. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:231435)
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The targeting vector is designed to insert a single loxP site upstream of exon 1, and a loxP-flanked and FRT-flanked neomycin resistance (neo) cassette downstream of exon 1. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:231435)